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Matched antibody pairs are sets of antibodies that are known to be capable of detecting different epitopes on the same protein antigen; they can be used together for the capture and detection of a single antigen in an immunoassay.
A DNA sequence encoding the Human IL13 (AAK53823.1) (Met1-Asn132) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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A DNA sequence encoding the Mouse TRIM21 (NP_001076021.1) (Met1-Met462) was expressed with a N-terminal polyhistidine tag followed by an AVI tag.The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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A DNA sequence encoding the Cynomolgus IL1B (P79182) (Ala117-Ser268) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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A DNA sequence encoding the Human IL31 (NP_001014358.1) (Ser24-Thr164) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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A DNA sequence encoding the Human CXCR6 (O00574) (Met1-Leu342) was expressed, with a Flag tag at the N-terminus, a polyhistidine tag and a strep tag at the C-terminus.
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A DNA sequence encoding the Human TL1A / TNFSF15 (NP_005109.2) (Leu72-Leu251) was expressed with a N-terminal polyhistidine tag followed by an AVI tag.The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. This construct has a trimer design.
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A DNA sequence encoding the Human IGF2 (P01344-1)(Ala25-Glu91) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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A cell-permeable, reversible inhibitor of exocytosis (IC50 = 20 µM); interferes with protein trafficking and secretion mediated by the Golgi apparatus and endoplasmic reticulum by dissociating the GTPase activator ARF1 from Golgi membranes
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A DNA sequence encoding the Human TNFSF13 (O75888-1) (Gln111-Leu250) was expressed with an N-terminal AVI tagged polyhistidine tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. This construct has a trimer design.
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